Abstract Detail

Systematics Section

Davis, Jerrold I. [1], Stevenson, Dennis Wm. [2], Ané, Cecile [3], dePamphilis, Claude W. [4], Givnish, Thomas J. [5], Graham, Sean W. [6], Leebens-Mack, James H. [7], Pires, J. Chris [8], Petersen, Gitte [9], Seberg, Ole [9], Thadeo, Marcela [10], Cuenca, Argelia [11], Ames, Mercedes [12], McNeal, Joel R. [7], Steele, P. Roxanne [13].

A phylogeny of the monocots based on plastid and mitochondrial gene sequences plus structural characters.

Two major goals of the Monocot Tree-of-Life project are to produce a detailed and strongly supported phylogeny of the monocots, and to identify structural features that are synapomorphies of major lineages. In order to accomplish the first of these goals, targeted sequencing of genes is being conducted for 600 monocot taxa (plus outgroups), and is complemented by whole plastid genome sequences from a subset of these taxa. The data matrix, at present, includes 250 taxa (190 monocots and 60 outgroups) scored for the plastid genes rbcL and matK, and the mitochondrial genes atp1 and nad5. Sequences of more than 75 additional genes also are available for 80 of these taxa. Analysis of this matrix yields four most-parsimonious trees and a correspondingly well resolved consensus tree, with one polytomy (within a family). The structure of the tree is consistent in most respects with previously published monocot phylogenies. The four targeted genes, and the remaining molecular data for the subset of 80 taxa, all provide critical support for the overall tree, thereby validating the sampling strategy. Analysis of a combined matrix that includes the molecular data and a set of structural characters yields similarly resolved trees. Characters marking major clades include root hair origin, presence/absence of vessels in stems, presence and form of silica accumulation, and variations in stomata, nectaries, endothecial wall thickenings, and pollen ultrastructure.

Broader Impacts:

1 - Cornell University, L.H. Bailey Hortorium, Department of Plant Biology, Ithaca, New York, 14853, USA
2 - New York Botanical Garden, 2900 Southern Boulevard, Bronx, NY, 10458, USA
3 - University of Wisconsin-Madison, Department of Botany, 430 Lincoln Drive, Madison, WI, 53706
4 - Pennsylvania State University, Department of Biology, 403 Life Sciences Building, University Park, Pennsylvania, 16802, USA
5 - University of Wisconsin-Madison, Department of Botany, Birge Hall, 430 Lincoln Dr., Madison, WI, 53706, U.S.A.
6 - University of British Columbia, Botanical Garden And Centre For Plant Research, 6804 Sw Marine Drive, Vancouver, British Columbia, V6T 1Z4, Canada
7 - University of Georgia, Department of Plant Biology, 4504 Miller Plant Sciences, Athens, GA, 30602, USA
8 - University of Missouri Columbia, Biological Sciences, 1201 Rollins Road, Life Sciences Center 311, Columbia, Missouri, 65211, USA
9 - Natural History Museum of Denmark, Sølvgade 83, Opg. S, Copenhagen, DK-1307, Denmark
10 - New York Botanical Garden, Science, 200th Street and Southern Blvd., Bronx, NY, 10458, USA
11 - Natural History Museum of Denmark, Sølvgade 83, Opg. S,, Copenhagen, DK-1307, Denmark
12 - University of Wisconsin - Madison, Horticulture, 1575 Linden Dr, Madison, Wisconsin, WI 53706, USA
13 - University of Missouri, Department of Biological Sciences, Columbia, MO, 65211, USA

Keywords:
monocots
phylogenetics
plastid genome
mitochondrial genome
morphology
matK
rbcL
atp1
nad5.

Presentation Type: Oral Paper:Papers for Sections
Session: 6
Location: 555A/Convention Center
Date: Monday, August 2nd, 2010
Time: 8:30 AM
Number: 6001
Abstract ID:638