Abstract Detail

Phytochemical Section

Gaviria-Velasquez, Mauricio [1], Quijano, Clara E. [2], Pino, Jorge A. [3], Madriñán, Santiago [1].

Chemical composition and antibacterial activity of the essential oil of Drimys granadensis L.f. from Colombia.

Winteraceae are a family of tropical and subtropical trees and shrubs present in Madagascar, Asia, Oceania, Central and South America. About 8 genera and more than 120 species have been reported for this family. The main chemical compounds found in this family are alkaloids, terpenes, oxygenated terpenes, antioxidants and flavonoids. Drimys is one of most representative genus of this family and is known as canelo (=cinnamon) in Latin America. It comprises about 58 species distributed along Central and South America over 2000 m elevation. This genus has been traditionally used as intoxicating narcotic, used to treat several ailments as pains, scurvy, gastrointestinal disorders, toothache, cancers, diarrhea, inflammations, affections of respiratory tract, and as a substitute for quinine in treating malaria and other fever conditions.
Leaves of Drimys granadensis L.f. were collected in Quebrada La Vieja Natural Reserve in Bogotá, Colombia; voucher specimen are deposited in ANDES. Oils were extracted by hydrodistillation using standard protocols. The oil yields were 0.1%. The composition of the essential oils from leaves of D. granadensis, was analyzed by GC and combined GC/MS with an HP 6890 GC Series II with a FID, equipped with an HP-5MS fused silica column. Oven temperature was programmed. Linear retention indices were calculated using n-paraffin standards. Compounds were identified by comparing their spectra to those of the Wiley and Nist libraries or our FLAVORLIB library and also by comparison of their GC retention index to those of standard compounds and data from literature. Of 92 signals detected, 89 compounds were totally or partially identified. The main compounds found were Germacrene D (14.7%), Sclarene (9.5%), α-Cadinol (7.3%), and (Z)-β-Ocimene (4.2%). The essential oil was also tested against 8 bacteria using the Kirby-Bauer Agar diffusion method. The Gram-positive bacteria were the most sensitive of the bacteria tested.

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Laboratorio de Botánica y Sistemática

1 - Universidad de los Andes, Departamento de Ciencias Biológicas, Laboratorio de Botánica y Sistemática, Apartado Aéreo 4976, Bogotá, DC, Colombia
2 - Universidad de los Andes, Departamento de Química, Laboratorio de Ciencias Agroalimentarias, Bogotá, DC, Colombia
3 - Instituto de Investigaciones para la Industria Alimenticia, Carrera del Guatao, Km 3.5, La Havana, 19200, Cuba

Keywords:
Drimys granadensis
Essential Oils
Antibacterial
Germacrene D.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Hall A/Convention Center
Date: Monday, August 2nd, 2010
Time: 5:30 PM
Number: PPT002
Abstract ID:88