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Abstract Detail

Conference Wide

Liston, Aaron [1].

An introduction to next-generation sequencing.

Next generation sequencing describes technologies that produce billions of base pairs of sequence data per experiment, at a cost of less than a dollar per megabase. Many universities have acquired next-generation sequencing technology in the past two years, but few plant biologists are aware of how to utilize this in their research. A major goal of the workshop will be to give participants enough information so that they can develop and initiate their own projects in phylogenetics, population genetics, and gene expression using this technology. In the morning, we will describe the available technologies, applications, and protocols for sample preparation and sequencing. We will also present strategies such as multiplexing for reducing costs. In the afternoon, we will describe options for computational infrastructure and the analytical pipeline. Participants will then have an opportunity to work with provided data, and generate assembled sequences. Participants will need to bring their own laptop for the "hands on" part of the workshop. We will email programs and data to install prior to the workshop.

Broader Impacts:

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1 - Oregon State University, Department of Botany & Plant Pathology, 2082 Cordley Hall, Corvallis, Oregon, 97331-2902, USA

none specified

Presentation Type: Activities:Workshops
Session: WS05
Location: 555/Convention Center
Date: Sunday, August 1st, 2010
Time: 9:00 AM
Number: WS05001
Abstract ID:1045

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