Cameron, Kenneth M. .
DNA Barcoding the Trees of Wisconsin.
A checklist was compiled for the native and naturalized trees located within the state of Wisconsin in the upper Midwest of the USA. Wisconsin represents a unique area for floristic study because it is the southern limit of the boreal forest, northern limit of the mixed deciduous forest, eastern limit of the prairie biome, western limit of the Great Lakes, and offers a mosaic of glaciated and unglaciated terrain dotted by terrestrial and aquatic habitats. Approximately 150 species of trees in 57 genera are known in the state (ca. 20% of the North American tree flora). Three individual specimens from each of these species were sampled randomly from the collection of the Wisconsin State Herbarium (WIS). Genomic DNA was extracted using a standard procedure and a fragment of rbcL was amplified in order to assay the utility of herbarium material and universal primers for DNA barcoding. Results are preliminary but encouraging in that most samples produced a quality sequence with minimal effort, and GenBank BLAST queries identified the samples at least to genus, if not to species in most cases, based on this 550 bp fragment alone. A two-gene barcode involving rbcL+matK is expected to be even more precise. DNA barcoding may be unable to identify all plant species when a worldwide monographic approach is undertaken for a taxon, but its success as an identification tool is high when used in a floristic context, especially within a finite geographic space.
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1 - Wisconsin State Herbarium, Department of Botany, University of Wisconsin, Madison, 430 Lincoln Dr., Madison, WI, 53706, USA
Presentation Type: Oral Paper:Papers for Sections
Location: 551A/Convention Center
Date: Monday, August 2nd, 2010
Time: 3:00 PM