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Abstract Detail

Physiological Section

Etxeberria, Ed [1], GonzŠlez, Pedro [1], Pozueta-Romero, Javier [2].

Retrograde vesicles from the vacuole regulate tonoplast surface area and volume during fluid-phase endocytosis.

During transport of external solutes from the apoplast to the vacuole by fluid-phase endocytosis (FPE), endocytic vesicles merge with the tonoplast to release their contents. The merging of endocytic vesicles with the vacuole results in a larger surface area/volume ratio than when separated, resulting in increase volume and dilution of vacuolar contents. To concentrate solutes in the vacuole, a mechanism(s) to reduce tonoplast surface area must exist. We demonstrated the existence of retrograde vesicles during FPE in red beet hypocotyls as recognized by their red and fluorescent vacuolar pigment, which identified any structure of vacuolar origin. In addition, fluorescent immunohistochemistry revealed that a 2% increase in vacuolar volume from endocytic fluids was accompanied by a 14% increase in tonoplast surface area.

Broader Impacts:

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1 - University of Florida, Citrus Research Center, 700 Experiment Station Road, Lake Alfred, FL, 33850, USA
2 - Agrobioteknologiako Instituta, Consejo Superior de Investigaciones Cientificas, Mutiloako etorbidea zembaki gabe, Mutiloabeti, Nafarroa, 31192, Spain

retrograde vesicles
tonoplast surface area.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Hall A/Convention Center
Date: Monday, August 2nd, 2010
Time: 5:30 PM
Number: PPS002
Abstract ID:339

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