Etxeberria, Ed , GonzŠlez, Pedro , Pozueta-Romero, Javier .
Retrograde vesicles from the vacuole regulate tonoplast surface area and volume during fluid-phase endocytosis.
During transport of external solutes from the apoplast to the vacuole by fluid-phase endocytosis (FPE), endocytic vesicles merge with the tonoplast to release their contents. The merging of endocytic vesicles with the vacuole results in a larger surface area/volume ratio than when separated, resulting in increase volume and dilution of vacuolar contents. To concentrate solutes in the vacuole, a mechanism(s) to reduce tonoplast surface area must exist. We demonstrated¬†the existence of retrograde vesicles during FPE in red beet hypocotyls as¬†recognized by their red and fluorescent vacuolar pigment, which identified any structure of vacuolar origin. In addition, fluorescent immunohistochemistry¬†revealed that a 2% increase in vacuolar volume from endocytic fluids was accompanied by a 14% increase in tonoplast surface area.
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1 - University of Florida, Citrus Research Center, 700 Experiment Station Road, Lake Alfred, FL, 33850, USA
2 - Agrobioteknologiako Instituta, Consejo Superior de Investigaciones Cientificas, Mutiloako etorbidea zembaki gabe, Mutiloabeti, Nafarroa, 31192, Spain
tonoplast surface area.
Presentation Type: Poster:Posters for Sections
Location: Hall A/Convention Center
Date: Monday, August 2nd, 2010
Time: 5:30 PM