Developmental and Structural Section
Mohamed-Yasseen, Yasseen .
Callus Formation and Plant Regeneration from Perianthes and Root Explants from Gladiolus X grandiflorus.
A procedure for plant regeneration from perianthes, different explants of gladiolus inflorescence and roots is described. Perianthes from different stages of development were cultured on Murashige and Skoog medium (MS) supplemented with 2 mg/l benzyladenine (BA) and 0.1 mg/l naphthaleneacetic acid (NAA). Small perianthes were cultured on MS containing 0.0, 1, 2, 3, 4, 5, 6 mg/l BA and 0.1 mg/l NAA. Inflorescence stem, inflorescence nodes, bracts and ovaries were cultured on MS supplemented with 2 mg/l BA and 0.1 mg/l NAA. Inflorescence explants and root segments were also cultured on callus-induction medium composed of MS supplemented with 1.5 mg/l dichlorophenoxyacetic acid (2,4-D), 0.5 mg/l NAA, 0.5 mg/l kinetin, 200 mg/l casein hydrolysate and 300 mg/l glutamine for callus induction. Multiple shoots were regenerated from all types of explants cultured on media containing BA and NAA. Shoot regeneration from perianthes was greatly affected by flower age and concentration of BA and NAA. Plantlets were regenerated from callus upon transfer to MS containing 0.2 mg/l BA. Produced shoots were transferred to root-induction medium composed of MS containing 0.1 mg/l indolebutyric acid (IBA). Rooted plantlets were transferred to soil and normal phenotypic plants were successfully established in soil.
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1 - Genetic Engineering & Biotechnology Research Institute- University of , Plant Biotechnology, Sadat
Presentation Type: Poster:Posters for Sections
Location: Hall A/Convention Center
Date: Monday, August 2nd, 2010
Time: 5:30 PM