jehan, Tabassum , lakhanpaul, Suman .
Evaluation of the genetic relationships among the members of family Hyacinthaceae in India.
To infer the genetic relationships among the members of Hyacinthaceae in India ITS and cpDNA were employed. ITS region amplified in eight species belonging to Dipcadi and Ledebouria gave an amplification product of 780 bp. In UPGMA dendrograms, two genera showed monophyletic grouping. The sequence alignment of ITS sequence revealed 819 sites and the cladogram showed lower homoplasy. ITS could not resolve the species relationships and showed intragenomic heterogeneity as clones of the same individual were not placed together in the cladogram. Three noncoding region of cpDNA namely, trnL intron, trnL-trnF spacer and psbA-trnH spacer were amplified in 41 accession belonging to 13 species of Drimia, Dipcadi and Ledebouria. The trnL-trnF and psbA-trnH spacer amplified products of 500 bp and 700 bp, respectively. Twelve locus-enzyme combinations resulted in 38 haplotypes. In dendrogram based on PCR-RFLP, the three genera showed monophyletic grouping corresponding to their subfamilies. Sequences of psbA-trnH spacer region of twelve species belonging to Drimia and Dipcadi revealed 672 sites out of which 32.5% sites were variable while 9.8% sites were found to be parsimoniously informative. In the cladogram Drimia indica, Drimia polyantha, Drimia wightii and Drimia coromandaliana showed close relationship while Drimia razii appeared diverged. Dipcadi saxorum and Dipcadi concanense showed affinity with each other and Dipcadi minor, Dipcadi montanum, Dipcadi ursulae and Dipcadi serotinum grouped in one clade. Dipcadi erythreum appeared to be most distinct. Sequences of trnL intron region from thirteen species belonging to Drimia, Ledebouria and Dipcadi from India as well as 25 taxa of Hyacinthaceae from Africa and Mediterranean region revealed 750 sites. Sequences of trnL –trnF spacer from thirteen species belonging to Drimia, Ledebouria and Dipcadi from India and 24 taxa of Hyacinthaceae from Africa and Mediterranean region revealed 532 sites and the region was found to contain five major indels. The indels showed interesting trends that will be discussed in the presentation.
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1 - University of Delhi, Botany, Delhi, India
2 - University of Delhi, Botany, Delhi, India
Presentation Type: Oral Paper:Papers for Sections
Location: 555A/Convention Center
Date: Monday, August 2nd, 2010
Time: 10:45 AM