Fay, Michael F. , Micheneau, Claire , Duffy, Karl .
Genetic variability in widespread Cephalanthera species (Neottieae, Orchidaceae) with different reproductive systems.
Plastid microsatellite loci developed for Cephalanthera longifolia were used to examine the level of genetic variation within and between populations of the three widepread Cephalanthera species (C. damasonium, C. longifolia, and C. rubra). The most detailed sampling was in C. longifolia (37 localities from Ireland-China; 128 individuals). Eight haplotypes were detected. One was detected in the vast majority of individuals and occurred from Ireland to Iran. Three others were only found in Europe (Ireland-France, England-Austria, and Austria-Croatia). Two were only found in the Middle East and two only in Asia. In C. damasonium, 20 individuals from nine populations (England-Turkey) were sampled. Only one haplotype was detected. In C. rubra, 33 individuals from eight populations (England-Turkey) were sampled. Although it was not possible to amplify all loci for all samples, 10 haplotypes were detected. Short alleles in two populations were due to deletions in the regions flanking the microsatellites. At this level of sampling, it appears that C. rubra has the greatest genetic variability. Cephalanthera longifolia, C. rubra, and C. damasonium have previously been characterized as outbreeding, outbreeding with facultative vegetative reproduction, and inbreeding, respectively. Patterns of genetic variation here are discussed in light of these reproductive system differences. The primers used in these three species of Cephalanthera were also demonstrated to amplify these loci in another five species. Although it is sometimes treated as a synonym of C. damasonium, C. yunnanensis from China had a different haplotype than that found in C. damasonium. All three loci were successfully amplified in two achlorophyllous, myco-heterotrophic species, C. austiniae and C. calcarata.
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1 - Royal Botanic Gardens, Kew, Jodrell Laboratory, Richmond, Surrey, TW9 3AB, United Kingdom
2 - Université Libre de Bruxelles, Evolutionary Biology and Ecology, 1050 Brussels, Belgium
3 - University of KwaZulu-Natal, School of Biological and Conservation Sciences, Private Bag X01, Scottsville, Pietermaritzburg , 3209, South Africa
Presentation Type: Oral Paper:Papers for Topics
Location: 556A/Convention Center
Date: Tuesday, August 3rd, 2010
Time: 10:45 AM