Ilut, Daniel , Doyle, Jeff J. .
Selecting nuclear sequences for fine detail molecular phylogenetic studies: a computational approach.
When studying the phylogenetic relationships of closely related species, it is often difficult to find nuclear DNA regions that are both easy to amplify across taxa and contain informative characters. In oder to address this problem, we have created a database of gene sequences specifically selected to greatly increase both the likelihood of amplification across the species of interest and the likelihood of retrieving nuclear regions that are variable among these species. This result is achieved by designing primers flanking putative intron splicing sites within highly conserved genes. Over 40 species were sampled spanning rosids (14 taxa), asterids (12 taxa), grasses (7 taxa) and other angiosperms, as well as several gymnosperms, a moss, and a green algae.
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Website listing low-copy, putatively intron-containing nuclear genes
1 - Cornell University, Department of Plant Biology, L.H. Bailey Hortorium, 412 Mann Library Building, Ithaca, NY, 14853-5908, USA
2 - Cornell University, Department of Plant Biology, L.H. Bailey Hortorium, 412 Mann Library Building, Ithaca,, New York, 14853-5908, USA
low-copy nuclear gene
Presentation Type: Oral Paper:Papers for Sections
Location: 555A/Convention Center
Date: Tuesday, August 3rd, 2010
Time: 3:30 PM