Cao, Daniel , Hauk, Warren .
An ISSR Investigation of North American grapefern species (Sceptridium; Ophioglossaceae).
In North America the center of Sceptridium species diversity is in the eastern half of the continent. Unlike closely related Botrychium s.s., in Sceptridium species concepts are poorly investigated, perhaps due to high amounts of intraspecific morphological variation and a paucity of species-specific characters. We investigated six diploid North American Sceptridium species (S. dissectum, S. oneidense, S. biternatum, S. multifidum, S. rugulosum, and S. lunarioides) using inter-simple sequence repeats (ISSRs) to test species concepts. Geographically representative samples from each species were amplified using a total of 9 ISSR primers. A mean of 28.8 loci per primer was generated, and nearly 100% of loci were polymorphic. Although preliminary Neighbor Joining and Maximum Parsimony analyses revealed most accessions of the same species clustered together, few fixed species-specific markers were detected. These six Sceptridium species may represent taxa that have not yet completed primary divergence, and this may help explain the difficulty of distinguishing species morphologically.
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1 - Denison University, Biology, Talbot Hall, 350 Ridge Road, Granville, OH, 43023, USA
2 - Denison University, Department of Biology, 100 W College Street, Granville, Ohio, 43023, USA
Presentation Type: Oral Paper:Papers for Sections
Location: 556B/Convention Center
Date: Monday, August 2nd, 2010
Time: 10:30 AM