Baker, Stokes S. .
Undergraduates developing a rapid cycling Brassica rapa transformation protocol.
Rapid cycling Brassica rapa (a.k.a., Wisconsin Fast Plantstm) has become an important resource for K-12 education. Unfortunately, an established transformation protocol is not available. As part of an inquiry assignment in the Plant Form and Function Laboratory course at the University of Detroit Mercy, undergraduates are attempting to develop an Agrobacterium-based floral dip transformation protocol. Results of the studentsí dosage response experiments have shown that both kanamycin (25 mg/L) and glufosinate (12.5 mg/L) can be used to select for transformants. Agrobacterium vacuum infiltration treatments were lethal. Fortunately, dipping plants in Agrobacterium suspensions containing the surfactant Silwet L-77 did not produce any visible deleterious effects. The treated plants produced good seed sets. The results of the studentsí final transformant screening will be presented. This project was funded through an Internal Research Fund grant provided by the University of Detroit Mercy.
By developing a transformation procedure for rapid cycling Brassica rapa, new K-12 inquiry curriculum can be developed. For example, by using inexpensive digital cameras, elementary school students can conduct experiments on plants expressing green fluorescent protein.
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Detecting GFP expression with consumer digital cameras
1 - University of Detroit Mercy, Biology Department, 4001 W. McNichols Road, Detroit, MI, 48221, USA
floral dip transformation
Wisconsin Fast Plants.
Presentation Type: Oral Paper:Papers for Sections
Location: 554B/Convention Center
Date: Tuesday, August 3rd, 2010
Time: 3:45 PM