Abstract Detail

Conservation Biology

Won, Hyosig [1], Kim, Joo Hwan [2], Lee, Jungho [3].

Genetic diversity and conservation issues of Euryale ferox Salisb. (Nymphaeaceae) in South Korea.

Euryale ferox Salisb. (Nymphaeaceae) is a herbaceous aquatic species distributed from India to Russian Far East, including China, Taiwan, Japan, and Korea. In South Korea, E. ferox is protected by law as an endangered species due to threats of destruction and reduction of habitats. To estimate the genetic diversity and present conservation strategy for the E. ferox populations in South Korea, we have collected 557 individuals from 37 sites across South Korea. Sequence variation of seven plastid regions – psbA-trnH IGS, psbK-psbI IGS, atpF-atpH IGS, trnL intron, trnL-trnF IGS, rpoC1 gene, and rpoB gene – were examined for the representatives of 20 populations, of which the psbA-trnH IGS and the psbK-psbI IGS regions only showed variation. The psbK-psbI IGS region showed two variations, an A9/A10 homopolymer and an A/G substitution, which comprised three haplotypes: A9/A, A9/G, and A10/G. We sequenced all the 557 samples for the psbK-psbI IGS regions and obtained 311 (56.8%) of A9/A, 20 (3.6%) of A9/G, and 217 (39.6%) of A10/G. Mostly, only one haplotype was observed per site, while two haplotypes co-occurred from eight sites. The A9/G-A10/G was observed from six sites, the A9/G-A10/G and the A9/A-A9/G from only one site, respectively. The A9/A haplotype dominated for 12 sites and the A10/G for 15 sites, respectively. The A9/G haplotype is the rarest of the three and occurred only in four sites along the western coast of South Korea. These results suggest that (1) there are at least three maternal founders for the E. ferox populations in Korea, (2) the lack of genetic diversity indicates that self-pollination is more common than cross-pollination (which is also indicated by the lack of chasmogamous flowers in the field), (3) those sites with the rarest haplotype A9/G have priority in conservation in Korea, and (4) further research using nuclear markers is necessary to unveil genetic structure of E. ferox.

Broader Impacts:

1 - Daegu University, Department of Biological Sciences, Jillyang, Gyungsan, Gyungbuk, 712-714, Korea (R.O.K.)
2 - Daegu University, Department of Biological Sciences, Gyungsan, Gyungbuk, 712-714, Korea (R.O.K.)
3 - Green Plant Institute, Inc., Bio Venture Valley, Seoul National University, Suwon, Gyeonggi, 441-853, Korea (R.O.K.)

Keywords:
Euryale ferox
Genetic variation
cp psbK-psbI IGS
conservation
cp haplotypes.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall A/Convention Center
Date: Monday, August 2nd, 2010
Time: 5:30 PM
Number: PCB010
Abstract ID:777